Project
Convergent deficits of memory consolidation in aged and sleep-deprived Drosophila
Sleep has been found to support memory consolidation in a spectrum of model organisms and the human being. With increasing organismal age, however, sleep fragments and memory consolidation attenuates. By which mechanisms and at what level the aging process intersects with memory consolidation and sleep remains largely unclear. In aging Drosophila fruit flies, consolidation of classical conditioned olfactory memory becomes ineficient and the number of sleep episodes increases. We in A08 will use this model to investigate this intersection concentrating on a brain-wide metaplastic change of synapses (increase of presynaptic active zone sizes) we recently found in aged flies. When we mimicked this meta-plasticity genetically (by elevating the gene dose of active zone scaffold proteins), memory consolidation decreased and the number of sleep episodes increased, similarly to aged flies. Here, we will reprogram presynaptic plasticity within relevant neuronal populations in order to learn about the synaptic and circuitry principles connecting aging, sleep and memory consolidation. Thus, we will measure how behavioral responses, such as olfactory aversive learning and sleep, are affected by opto−physiological methods combined with genetic manipulation. In addition, dorsal paired medial neurons will be specifically analyzed, as they have been previously shown to promote olfactory memory consolidation, and their artificial stimulation influences sleep and can compensate for age-induced deficits of consolidation. A08 will also use spermidine feeding as a protective paradigm, found to alleviate age-induced effects on memory consolidation. This strategy should help us to further separate causal entities from epiphenomena at the intersection of age, memory consolidation and sleep.
Team
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Prof Dr Stephan Sigrist
Freie Universität Berlin
Head Sigrist Lab (FU & Charité Berlin)
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Dr Oriane Turrel
Freie Universität Berlin
Postdoc A08
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Dr Sheng Huang
Freie Universität Berlin
Associated Postdoc A08
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David Toppe
Freie Universität Berlin
PhD A08
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Dr Yongtian Liang
Freie Universität Berlin
(A08 Alumnus)
Publications
Homeostatic active zone remodeling consolidates memories in the Drosophila mushroom body
Oriane Turrel, Niraja Ramesh, Marc J.F. Escher, Stephan J. Sigrist
bioRxiv (2021)
Presynaptic and postsynaptic long-term plasticity in sleep homeostasis
Sheng Huang, Stephan J. Sigrist
Curr Opin Neurobiol. 69:1-10 (2021)
Recruitment of release sites underlies chemical presynaptic potentiation at hippocampal mossy fiber boutons
Marta Orlando, Anton Dvorzhak, Felicitas Bruentgens, Marta Maglione, Benjamin R. Rost, Stephan J. Sigrist, Jörg Breustedt, Dietmar Schmitz
PLoS Biol. 19(6):e3001149 (2021)
Unc13A and Unc13B contribute to the decoding of distinct sensory information in Drosophila
Atefeh Pooryasin , Marta Maglione , Marco Schubert, Tanja Matkovic-Rachid, Sayed-mohammad Hasheminasab, Ulrike Pech, André Fiala, Thorsten Mielke, Stephan J. Sigrist
Nat Commun. 12(1):1932 (2021)
eIF5A-hypusination, boosted by dietary spermidine, protects from premature brain aging and mitochondrial dysfunction
YongTian Liang, Chengji Piao, Christine B. Beuschel, David Toppe, Laxmikanth Kollipara, Boris Bogdanow, ..... Stephan J. Sigrist
Cell Rep. 35(2):108941 (2021)
The Unc13A isoform is important for phasic release and olfactory memory formation at mushroom body synapses
Jennifer Woitkuhn, Anatoli Ender, Christine B Beuschel, Marta Maglione, Tanja Matkovic-Rachid, Sheng Huang, Martin Lehmann, Joerg RP Geiger & Stephan J Sigrist
J Neurogenet. 34(1):106-114 (2020)
Presynaptic activity zone plasticity encodes sleep need in Drosophila
Sheng Huang, Chengji Pia, Christine B Beuschel, Torsten Götz, Stephan J Sigrist
Curr Biol. 30(6):1077-1091.e5 (2020)
Network-specific synchronization of electrical slow-wave oscillations regulates sleep drive in Drosophila
Davide Raccuglia, Sheng Huang, Anatoli Ender, M.-Marcel Heim, Desiree Laber, Raquel Suárez-Grimalt, Agustin Liotta, Stephan J Sigrist, Jörg RP Geiger, David Owald
Curr Biol. 29(21):3611-3621.e3 (2019)
Emerging concepts and functions of autophagy as a regulator of synaptic components and plasticity
Yongtian Liang
Cells. 8(1):34 (2019)
Autophagy within the mushroom body protects from synapse aging in a non-cell autonomous manner
Anuradha Bhukel, Christine B Beuschel, Marta Maglione, Martin Lehmann, Gabor Juhász, Frank Madeo and Stephan J Sigrist
Nat Commun. 10(1):1318 (2019)