Differential embedding of hippocampal output neurons during memory-related oscillations

This project aims to study a potential microcircuit responsible for channeling hippocampal sharp-wave ripple events (SWRs, Buzsaki, 1989) via the subiculum into the cortical areas (Nitzan et al. 2020) for memory consolidation. To understand the functional role that a subtype of pyramidal cells within the CA1 play in memory consolidation, we will study the connectivity between area CA1 and the subiculum using a variety of optogenetic tools in vivo and in vitro. The main cellular subtype we will be focusing on are the Calbindin-containing superficial cells of the CA1. We will also study the propagation of SWRs on a network level using in vivo silicon probe recordings. Finally, we aim to understand the role of a potential microcircuit involving Calbindin-containing superficial CA1 cells and the subiculum in memory-related behaviors in animals.

Archive – A02 (SFB1315/1)

Graphical abstract: Hippocampal output neurons, such as Calbindin-containing CA1 pyramidal cells, could perform a specific role in memory-related oscillations. This project aims to study a potential microcircuit responsible for channeling hippocampal sharp-wave ripple events (SWRs, Buzsaki, 1989) via the subiculum (SUB) into the cortical areas (Nitzan et al. 2020) for memory consolidation. To understand the functional role the subtypes of pyramidal cells within the CA1 play in memory consolidation, we will study the connectivity between single cells in the CA1 and SUB using optogenetic tools in in vitro slices, then proceed to study the mechanisms controlling onset and termination of SWRs on a network level using in vivo probe recordings, and finally the role and effect of this microcircuit on memory-related behaviors in animals. Graphic includes images from SciDraw (https://scidraw.io/).