Project
Development of molecular tools for manipulating and studying memory engrams
Over the last decade the number, variety and specificity of optical and genetic tools that report or modify neural activity, such as calcium and voltage sensors or light-gated channels and light driven pumps, has increased exponentially. Many if not most laboratories now use genetically encoded tools to address research questions, however the accessibility of these tools remains limited. In this CRC, we will offer custom tailored viral vector based tools optimized for investigation of the mechanisms underlying memory consolidation. The choice of tools will take into account two main parameters: spatial confinement of such tools (i.e. the neurons and circuits involved), and their biophysical properties to match the experimental condition and the biology of memory consolidation. These tools have recently advanced beyond simple manipulation and now allow researchers within the CRC to manipulate, investigate or reconstruct neural circuits. How memory circuits are consolidated can also be studied by examining gene expression in the participating neurons. Correlating gene expression with connectivity patterns can be particularly useful in describing underlying mechanisms of memory formation, as well as providing anatomical markers for neurons participating in the memory engrams. This can be accomplished through the use of transcriptome analysis by means of single cell RNA sequencing.
Knowhow in molecular biology, cell biology, physiology and bioinformatics are required to develop the above tools, as well as analyzing and understanding the outcome of their use. The C01 project will serve as a hub for research in design and production of tools in order to support circuit analysis on morphological, molecular and functional levels.
Image CA1 hippocampal formation infected with pAAV-Syn-FLEX-Ce3ToG-WPRE3 (Cerulean, TVA receptor, Glycoprotein G) and with the Rabies virus pRVdG-N-P-M-EGFP-SynPhRFP-L (expressing green fluorescent protein and Synaptophysin-RFP). Courtesy of Dr. Julie Ledderose (AG Larkum)
Team
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Prof Dr Christian Rosenmund
Charité-Universitätsmedizin Berlin
Head Rosenmund Lab
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Prof Dr Dietmar Schmitz
Charité-Universitätsmedizin Berlin
Head Schmitz Lab
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Prof Dr Peter Hegemann
Humboldt-Universität zu Berlin
Head Hegemann Group
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Dr Thorsten Trimbuch
Charité-Universitätsmedizin Berlin
Head, Viral Core Facility (VCF)
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Dr Anisha Dayaram
Charité-Universitätsmedizin Berlin
Postdoc C01
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Claire Cooper
Charité-Universitätsmedizin Berlin
PhD C01
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Dr Matthias Broser
Humboldt-Universität zu Berlin
Postdoc C01
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Dr Beatriz Rebollo-Gonzalez
Charité-Universitätsmedizin Berlin
Associated Postdoc C01
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Dr Yinth Andrea Bernal
Humboldt-Universität zu Berlin
Associated Postdoc C01
Publications
Far-red absorbing rhodopsins, insights from heterodimeric rhodopsin-cyclases
Matthias Broser
Front Mol Biosci. 8:806922 (2022)
BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons
Johannes Vierock, Silvia Rodriguez-Rozada, Alexander Dieter, Florian Pieper, Ruth Sims, Federico Tenedini, Amelie C. F. Bergs, Imane Bendifallah, Fangmin Zhou, Nadja Zeitzschel, Joachim Ahlbeck, Sandra Augustin, Kathrin Sauter, Eirini Papagiakoumou, Alexander Gottschalk, Peter Soba, Valentina Emiliani, Andreas K. Engel, Peter Hegemann , J. Simon Wiegert
Nat Commun. 12(1):4527 (2021)
Creating detailed metadata for an R-shiny analysis of circadian behavior sequence data
Julien Colomb, York Winter
Front Neurosci (in press) 2021
Efficient optogenetic silencing of neurotransmitter release with a mosquito rhodopsin
M. Mahn, I. Saraf-Sinik, P. Patil, M. Pulin, E. Bitton, N. Karalis, F. Bruentgens, ..... D. Schmitz, A. Lüthi, B. R. Rost, J. S. Wiegert and O. Yizhar
Neuron. 109(10):1621-1635.e8 (2021)
Somatostatin interneurons activated by 5-HT2A receptor suppress slow oscillations in medial entorhinal cortex
Roberto de Filippo, Benjamin R Rost, Alexander Stumpf, Claire Cooper, John J Tukker, Christoph Harms, Prateep Beed, Dietmar Schmitz
eLife. 10:e66960 (2021)
SynaptoPAC, an optogenetic tool for induction of presynaptic plasticity
Silvia Oldani, Laura Moreno‐Velasquez, Lukas Faiss, Alexander Stumpf, Christian Rosenmund, Dietmar Schmitz, Benjamin R Rost
J Neurochem. 156(3):324-336 (2021)
Layer 6b is driven by intracortical long-range projection neurons
Timothy A. Zolnik, Julia Ledderose, Maria Toumazou, Thorsten Trimbuch, Tess Oram, Christian Rosenmund, Britta J. Eickholt, Robert N.S. Sachdev, Matthew E. Larkum
Cell Rep. 30(10):3492-3505.e5 (2020)
Generation of sharp wave-ripple events by disinhibition
Roberta Evangelista, Gaspar Cano, Claire Cooper, Dietmar Schmitz, Nikolaus Maier and Richard Kempter
J Neurosci. 40(41):7811-7836 (2020)
Membrane bridging by Munc13-1 is crucial for neurotransmitter release
Bradley Quade, Marcial Camacho, Xiaowei Zhao, Marta Orlando, Thorsten Trimbuch, Junjie Xu, Wei Li, Daniela Nicastro, Christian Rosenmund
eLife. 8:e42806 (2019)