Project
Inhibitory control of memory consolidation in primary somatosensory cortex
Recent evidence suggests that our ability to memorize sensory events relies on activity in primary sensory cortices in the hours after training, but the cellular mechanisms are unclear. Our hypothesis is that local inhibition shapes the offline replay of activity in excitatory neurons and supports the formation of cellular engrams. To test this hypothesis, we aim to use a rapidly learnt thermal stimulus detection task in combination with activity manipulations and longitudinal two-photon calcium imaging. We have four work packages; (i) testing the role of fS1 in a novel thermal detection task; investigate the global and local activity of the fS1 (ii) excitatory and (iii) inhibitory networks pre-, during and post- learning; (iv) quantify the impact of disinhibitory pathways on consolidation. Our project will contribute to the CRC by investigating the mechanisms of memory consolidation at the level of identified cell types embedded within connected circuits in vivo.
Image Thermal image of mouse with forepaw resting on Peltier element (See also Neuron 106(5)830-841. 2020). Courtesy of Ricardo Paricio-Montesinos
Team
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Prof Dr James Poulet
Max-Delbrück-Centrum für Molekulare Medizin
Head Poulet Lab
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Prof Dr Benjamin Judkewitz
Charité-Universitätsmedizin Berlin
Head Judkewitz Lab - A05 Alumnus PI
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Dr Clarissa Whitmire
Max-Delbrück-Centrum für Molekulare Medizin
A05 Associate Postdoc
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Dr Annapoorani Udhayachandran
Max-Delbrück-Centrum für Molekulare Medizin
A05 Associate Postdoc
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Dr Mikkel Vestergaard
Max-Delbrück-Centrum für Molekulare Medizin
A05 Associate Postdoc
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Ricardo Paricio-Montesinos
Charité-Universitätsmedizin Berlin
PhD (A05 Alumnus)
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Tobias Leva
Max-Delbrück-Centrum für Molekulare Medizin
PhD (A05 Alumnus)
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Caroline Berlage
Charité-Universitätsmedizin Berlin
(A05 Alumnus)
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Ian Erik Meredyth Stewart
Max-Delbrück-Centrum für Molekulare Medizin
(A05 Alumnus)
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Nina-Franziska Stephan
(A05 Alumna) Student helper 2021
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Bibiana Horn
(A05 Alumna) Student helper 2020
Publications
The cellular coding of temperature in the mammalian cortex
Mikkel Vestergaard, Mario Carta, Gamze Güney, James F.A. Poulet
Nature 614, 725–731 (2023)
Deep tissue scattering compensation with three-photon F-SHARP
Caroline Berlage, Malinda Tantirigama, Mathias Babot, Diego Di Battista, Clarissa Whitmire, Ioannis N. Papadopoulos, James F. A. Poulet, Matthew Larkum, Benjamin Judkewitz
Optica. 8:1613-1619 (2021)
USH2A is a Meissner’s corpuscle protein necessary for normal vibration sensing in mice and humans
Fred Schwaller, Valérie Bégay, Gema García-García, Francisco J. Taberner, Rabih Moshourab, Brennan McDonald, Trevor Docter, Johannes Kühnemund, Julia Ojeda-Alonso, Ricardo Paricio-Montesinos, Stefan G. Lechner, James F. A. Poulet, Jose M. Millan, Gary R. Lewin
Nat Neurosci. 24(1):74-81 (2021)
Perspective on the multiple pathways to changing brain states
Malinda LS Tantirigama, Timothy Zolnik, Benjamin Judkewitz, Matthew E. Larkum and Robert NS Sachdev
Front Syst Neurosci. 14:23 (2020)
The sensory coding of warm perception
Ricardo Paricio-Montesinos, Frederick Schwaller, Annapoorani Udhayachandran, Florian Rau, Jan Walcher, Roberta Evangelista, Joris Vriens, Thomas Voets, James FA Poulet, Gary R. Lewin
Neuron. 106(5):830-841.e3 (2020)
Dynamic conjugate F-SHARP microscopy
Ioannis N Papadopoulos, Jean-Sebastien Jouhanneau, Naoya Takahashi, David Kaplan, Matthew Larkum, James Poulet and Benjamin Judkewitz
Light Sci Appl. 9:110 (2020)
Multiple two-photon targeted whole-cell patch-clamp recordings from monosynaptically connected neurons in vivo
Jean-Sébastien Jouhanneau and James FA Poulet
Front Synaptic Neurosci. 11:15 (2019)